Soybean transformation by Agrobacterium has been very well established to produce good transformation efficiency. The most often method used is the cotyledon node method which produces consistent good quality transgenic events with low copy numbers while giving good integration of the TDNA. We used protocol (Quach et al. 2024) to successfully generate transgenic soybean having 2-9 expression cassettes to study the metabolic pathways of long chain unsaturated fatty acids and other nutrition soybean genetic engineering.
PlantEdits delivers T0 plant transgenic plantlets after they are established therefore researchers will need some experience with taking care of soybean in greenhouse as the primary transgenic event are often smaller and produce fewer seeds. In the future with higher demand, we will offer shipping the T1 seeds for most of our clients.
The total time required is 4-6 months to retrieve the T0 plantlets. The transgenic plants are confirmed by either leaf painting or PCR. We apply herbicide painting on multiple leaves to make sure minimum chimeric occurrence rate.
For the service charge, we offer competitive pricing, and we have a low introductory rate. For customers who request greater number of events (more than 10) or large TDNA, please contact us to discuss about discount or additional service charge.
As we have been successful in transformation of a wide selection of soybean genotypes, we are able provide service to transform elite breeding lines that allow breeders to incorporate useful traits to the future release soybean cultivars.
For genome editing. We are currently working with UNL innovation campus to rent greenhouse space. We expect to offer the service in 2026 to provide edited plants to customers.
Quach, T., Nguyen, H., Meyer, O., Sato, S.J., Clemente, T.E. and Guo, M., 2023. Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.). In Plant Genome Engineering: Methods and Protocols (pp. 273-285). New York, NY: Springer US.
