Maize transformation utilizes immature embryonic tissues. The Agrobacterium-based method has been well established for Hi-II, B104 and LH244 and produces good quality transgenic events with very low chimeric rate. Our protocol has produced transgenic events for improved nutrition quality, herbicide resistance, and biological process studies (Hurst et al. 2024).
Currently we are sending T0 plantlets to customers since we have limited capacity to generate T1 seeds. Typical maize transformation process from bacterial inoculation to the plantlet stage is about 3-4 months. We use antibiotic selection for the presence of nptII selectable marker and perform ELISA test for the npt-II protein. We can help perform PCR with customers provided primers for the genes of interest.
For the service charge, we offer competitive pricing, and we have a low introductory rate for B104, LH244 and H99.
Please refer to the pricing page for more information. For customers who request a greater number of events (more than 10), long TDNA or larger number of vectors, please contact us to discuss discounts or additional service charges.
For genome editing. We are currently working with UNL innovation campus to rent greenhouse space. We expect to offer the service in 2026 to provide edited plants to customers.
References
Hurst, J. Preston, et al. “Editing the 19 kDa alpha‐zein gene family generates non‐opaque2‐based quality protein maize.” Plant Biotechnology Journal 22.4 (2024): 946-959.