Wheat transformation utilizes immature embryonic tissues with a preculture period. The method has been
well established and produces good quality transgenic events with single copy number of ~50%. Our protocol
has produced transgenic events for improved stress tolerance, virus resistance, nitrogen use efficiency and
other agronomic traits (Pena et al. 2017, Tatineni et al. 2020, Placido et al. 2020).
Currently we are sending T0 plantlets to customers since we have limited capacity to generate T1 seeds.
Typical wheat transformation process from bacterial inoculation to the plantlet stage is about 4-5 months. We
use antibiotic selection for the presence of nptII selectable marker and perform ELISA test for the npt-II
protein. We can help perform PCR with customers provided primers for the genes of interest.
For the service charge, we offer competitive pricing, and we have a low introductory rate for CBO37. Please
refer to the pricing page for more information. For customers who request a greater number of events (more
than 10), long TDNA or larger number of vectors, please contact us to discuss discounts or additional service
charges.
For genome editing. We are currently working with UNL innovation campus to rent greenhouse space. We
expect to offer the service in 2026 to provide edited plants to customers.
References
Peña, P.A., Quach, T., Sato, S., Ge, Z., Nersesian, N., Changa, T., Dweikat, I., Soundararajan, M. and Clemente,
T.E., 2017. Expression of the maize Dof1 transcription factor in wheat and sorghum. Frontiers in Plant
Science, 8, p.434.
Tatineni, S., Sato, S., Nersesian, N., Alexander, J., Quach, T., Graybosch, R.A. and Clemente, T.E., 2020.
Transgenic wheat harboring an RNAi element confers dual resistance against synergistically interacting wheat
streak mosaic virus and Triticum mosaic virus. Molecular Plant-Microbe Interactions, 33(1), pp.108-122.
Placido, D.F., Sandhu, J., Sato, S.J., Nersesian, N., Quach, T., Clemente, T.E., Staswick, P.E. and Walia, H., 2020.
The LATERAL ROOT DENSITY gene regulates root growth during water stress in wheat. Plant Biotechnology
Journal, 18(9), pp.1955-1968.